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KMID : 0895820020120030095
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Journal of Oriental Rehabilitation Medicine
2002 Volume.12 No. 3 p.95 ~ p.107
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Effects of Bujabanha-tang(Fuzibanxia-tang) Water Extract on the Cultured Spinal Sensory Neurons Damaged by H2O2
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Jung Sang-Pil
Choi Kyu-Sun
Lee Young-Bo
Seo Young-Suk
Kwon Young-Dal
Shin Byoung-Chul
Lee Su-Kyung
Song Yung-Sun
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Abstract
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Objectives and Methods: To evaluate the effect of Bujabanha-tang water extract on cultured mouse spinal sensory neurons which was inhibited by hydrogen peroxide (H2O2)-induced cytotoxicity, MTT assay, NR assay and neurofilament enzymeimmunoassay(EIA), LDH activity assay were carried out after the cultured mouse spinal sensory neurons were pre-incubated with various concentrations of Bujabanha-tang water extract for 3 hours prior to exposure of hydrogen peroxide.
Results: H2O2, an oxygen radical, decreased the survival rate of the cultured mouse spinal sensory neuron cells on NR assay and MTT assay. Bujabanha-tang water extract has efficacy of increasing neurofilament decreased by hydrogen peroxide and decreasing LDH activity increased by hydrogen peroxide in cultured mouse spinal sensory neurons.
Conclusions: From the above results, it is suggested that Bujabanha-tang has marked efficacy as a treatment for the damages caused in the H2O2-mediated oxidative process.
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KEYWORD
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Bujabanha-tang, Hydrogen Peroxide(H2O2), MTT, NR, Neurofilament enzymeimmunoassay(EIA), LDH activity
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